Western blot analysis of RBBP5 in different various lysates. Lane 1: SW480 cell lysate; Lane 2: A431 cell lysate; Lane 3: C2C12 cell lysate; Lane 4: 293 cell lysate; Lane 5: MCF-7 cell lysate; Lane 6: NIH/3T3 cell lysate; Lane 7: K562 cell lysate; Lane 8: N2A cell lysate; Lane 9: PC-12 cell lysate Lane 10: RAW264.7 cell lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 59 kDa. Observed band size: 59 kDa. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Primary antibody RBBP5 recombinant monoclonal antibody (Product # MA5-50779) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.